SDS Removal
SDS is often present in proteins and peptides electroeluted from SDS-PAGE gels. It is also used to solubilize proteins during digestion. Unfortunately, the SDS in the samples can ruin subsequent runs in reversed-phase HPLC (RPC) and mass spectra. A guard cartridge of our SDS Removal Material, screwed into your RPC column, will successfully remove the SDS from peptide samples. With conventional conditions of acetonitrile (ACN) gradients in 0.1% TFA, it will retain the SDS but not the peptides. Accumulated SDS is washed off the cartridge (and out the RPC column) by levels of ACN higher than 70%.
As an alternative approach, or if a sample contains an unusually high level of SDS, one can remove SDS or any other detergent via hydrophilic interactive chromatography (HILIC) with a TopTip® SPE cartridge of PolyHYDROXYETHYL A™
PolyHYDROXYETHYL A™ is a trademark of PolyLC Inc. All Rights Reserved
TopTip® are trademarks of Glygen Corp.
Click here to download SDS Removal Instruction Sheet
Click here to download MSDS Instruction Sheet
For technical problems with this website contact the webmaster at cwike@PolyLC.com
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